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	Provedor de dados ArchiMer (2) Autor Apaire, M (2) Besse, B (2) Billaudel, S (2) Leguyader, F (2) Palavra-chave Biology (1) DNA (1) DNA probes (1) Detection (1) Enterovirus (1) France (1) Hepatitis A virus (1) Loire Estuary Hepatitis A virus Enterovirus Bivalvia Hybridization analysis Seafood Estuaries Rivers Sensors RNA Disease detection Viruses Shellfish Biology (1) Loire R. ANE (1) Marine molluscs (1) Mollusca (1) Pays de Loire (1) Picornaviridae (1) RNA (1) RNA probes (1) Seafood (1) Sensors (1) Shellfish (1) Viruses (1) Mais... Tipo do documento Text (2) Ano 1992 (2) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish ArchiMer Apaire, M; Leguyader, F; Besse, B; Billaudel, S. The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes. Tipo: Text Palavras-chave: Mollusca; Enterovirus; Hepatitis A virus; Picornaviridae; RNA probes; DNA probes; Seafood; Biology; Sensors; DNA; RNA; Viruses; Detection; Marine molluscs; Shellfish. Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1613.pdf Evaluation by molecular biology of enterovirus and hepatitis A virus contamination of shellfish in the Loire River estuary region ArchiMer Leguyader, F; Apaire, M; Besse, B; Billaudel, S. Riboprobes developed in our laboratory were used for detection of enteroviruses and hepatitis A virus (HAV) in shellfish (cockles and mussels). After concentration elution, virus nucleic acids were extracted and purified. Hybridization was performed on a solid support according to the dot-blot technique, with (32P) UTP labelling of probes during transcription. Results show high shellfish contamination in samples: 63% enteroviruses and 67% HAV. Cockles were particularly contaminated: 89% enteroviruses and 84% HAV. Tipo: Text Palavras-chave: France; Loire R. ANE; France; Pays de Loire; Loire Estuary Hepatitis A virus Enterovirus Bivalvia Hybridization analysis Seafood Estuaries Rivers Sensors RNA Disease detection Viruses Shellfish Biology. Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1616.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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